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yard Negative Unknown Upon being given a bacteriuml elaboration containing two bacterium, I was assigned the task of keep a detonate the several(prenominal) bacteria. The two bacteria of the mixture were 1 one thousandme dogmatic and one gigabyte negative. The experiment has two split and this is the part of isolating the gee-negative bacteria. The technique used was called streaking for closing bump through in which the bacteria were streaked thinly on food for thought agar in different directions in a normal that left respective(prenominal) colonies on the final growth streak. These individual colonies could be identified by their individual characteristics such as color and shape and distinguished from one another. This was not tardily with this burnish because some(prenominal) bacteria grew in colonies that were small, white, and shaped similar. To sieve these bacteria I had to first gram stain the bacterial mixture to see if I had both types of bacteri a and so I used the following stairs to perform the gram stain. 1. The bacteria are discolourationed on a slide and allowed to modify. 2. The bacteria is heat fixed to the slide 3. crystallizing violet pigment is added to the smear for 1 minute 4. dry wash the pigment dark with distilled peeing 5. Apply Grams Iodine for 1 minute 6. Wash iodine off with distilled water 7.

Decolorize the smear with 95% ethyl souse beverage hurtle by drop until it runs off communicate 8. Wash off alcohol with distilled water 9. Counterstain with Safranin for 45 seconds 10. Wash off Safranin with distilled water 1 1. Blot dry with Bibulous paper Followin! g this test, I found that I had a lot more gram negative bacteria than the gram positive and so I could easily embrace one colony of my streaked husbandry and have a high chance of growing a pure culture from one of the isolated colonies. So, with this method, I grew a pure culture of the gram negative bacteria that I would use in my tests to determine its identity. I ran several tests on the bacteria utter(a) with the Gram stain that proved that the bacteria were gram negative....If you wish to get a full essay, couch it on our website: OrderCustomPaper.com

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